EVE 161 – Lecture 2 – Sequencing Revolution

History of Sequencing (with a focus on more recent developments)

Main reading:

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Outline and Notes

  1. Timeline of DNA sequencing
  2. Generation I: Manual sequencing
    1. Maxam – Gilbert
    2. Sanger
    3. Some details on Sanger
  3. Generation I+: Automation
    1. Capillary / fluorescent Sanger
    2. Colony picking robotics
    3. Highlights
  4. Generation II: Colonies not Clones

 

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