Well, this paper is generating a lot of buzz on social media. Nucleic acid purification from plants, animals and microbes in under 30 seconds.
For example see Eric Topol’s Tweet about it
— Eric Topol (@EricTopol) November 21, 2017
Citation: Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, et al. (2017) Nucleic acid purification from plants, animals and microbes in under 30 seconds. PLoS Biol15(11): e2003916. https://doi.org/10.1371/journal.pbio.2003916
Author summary: Nucleic acid amplification has proven to be indispensable in laboratories around the world for a myriad of applications from diagnostics to genotyping. The first step in any application aiming to amplify DNA or RNA is the extraction of nucleic acids from a complex biological sample; a task traditionally requiring specialised equipment, trained technicians, and multiple liquid handling steps. It is this complexity of current nucleic acid isolation methods that limit the use of many DNA amplification technologies outside of the modern laboratory environment. Therefore, in this study, we investigated new materials and approaches to simplify nucleic acid extraction. We found that cellulose-based filter paper can be used to rapidly bind nucleic acids, retain them during a short washing step to remove contaminants, and then elute them directly into the amplification reaction. We then adapted the cellulose filter to create a dipstick that can be used to purify nucleic acids from a wide range of plant, animal, and microbe samples in less than 30 seconds without the need for any specialised equipment. The speed and simplicity of our method makes it ideally suited for nucleic acid amplification-based applications both within and outside the laboratory, including limited resource settings such as remote field sites, developing countries, and teaching institutions.
Definitely seems interesting. However, it is unclear just how useful this will be for microbiome studies since they do not present anything about the effect of this protocol on microbial community analysis (e.g., maybe it only gets DNA from some taxa). Still – very interesting.