microBEnet journal club: MetaBAT for reconstructing single genomes from complex microbial communities

    There is an interesting paper out a few days ago in PeerJ: MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities. By Dongwan D. Kang, Jeff Froula, Rob Egan, Zhong Wang​.  The key to what they do in the paper is summarized in Figure 1:   The legend is below: There are three preprocessing steps before MetaBAT is …

Gut check: the ecology of keeping time

There are few constants in this world. One exception, however, is the passing of day to night, which has gone on without fail since life first emerged on Earth. Early life quickly learned to anticipate changes associated with light and dark. This ability to tell time – to peer into the immediate future – was …

Workshop to produce curated 18S rDNA reference database

Just got this announcement by email and I thought it might be of interest: Join the curation effort & enroll in our first workshop! What: Workshop to produce curated 18S rDNA reference database. When: July 19th — 25th, 2015. Where: Vancouver, Canada on the University of British Columbia campus. How much: We will cover the cost of housing, breakfast and …

CAMI Challenge is Now Open for Participation

Dear metagenome method developers, The first challenge of the Initiative for the Critical Assessment of Metagenome Interpretation (CAMI) begins right now! Over the last three months, we received valuable feedback from the community playing with our toy data sets. We incorporated many of your suggestions, thanks again! Today, we proudly release the official data sets …

SciPy 2015 Computational Life and Medical Sciences Mini-Symposium: Call for Abstracts

SciPy 2015 (Scientific Computing with Python) is coming up in Austin, TX this July 6-12. I attended SciPy last year for the first time to present on scikit-bio (see my talk here), and thought it was an excellent meeting. It was great to spend a week talking about software and software development, which isn’t the …

What sequencing core do you recommend for environmental samples with low concentrations?

Have you ever wondered what’s in the air of a daycare center, where kids swap microbes every day? A team from Virginia Tech and the University of Pittsburgh is investigating the viral ecology in air of a daycare center using metagenomics. We have collected samples on HVAC filters and are looking for a sequencing core …

New paper on diseased vs healthy infants in a NICU, possible impacts for future hospital microbiome work

This week in eLife, our lab published a study entitled Gut bacteria are rarely shared by co-hospitalized premature infants, regardless of necrotizing enterocolitis (NEC) development. Spearheaded by a talented Banfield Lab post-doc, Tali Raveh-Sadka, in collaboration with Michael Morowitz’s Lab, the study aimed to find the causative agent in an outbreak of NEC cases that …

Issues classifying ITS data? The answer could be simply using “blast” during taxonomy assignment in QIIME

I wrote a post on the Seagrass Microbiome website yesterday about my struggles with fungal ITS sequencing data which I thought I’d share here as well in case anyone is looking to jump into the fungal fray. To summarize: changing the default method of the QIIME assign_taxonomy.py script from “UCLUST” to “blast” dramatically increased the …