Comparing the new 16S rRNA V4 and ITS primers to the old primers-RESULTS!

The Knight lab has been working hard testing new primers for 16S rRNA amplicon production and its time to share our progress. So far, the 16S rRNA V4 region forward primer (designated 515f) has been paired with five different reverse primers (806r, 926r, 967r, 1048r, and 1391r) to amplify ribosomal RNA from bacteria, Archaea, and …

American Gut data and pipelines now freely available

While not exactly the microbiology of the built environment, studies of the human microbiome are the next closest thing and and understanding of both will be critical to creating healthier living environments. The American Gut project is the largest, crowdfunded/citizen science human microbiome effort, with over 3,000 participants so far (including myself).  In addition to …