This paper may be of use to people working on plant microbiomes. Peredo EL and Simmons SL (2018) Leaf-FISH: Microscale Imaging of Bacterial Taxa on Phyllosphere. Front. Microbiol. 8:2669. doi: 10.3389/fmicb.2017.02669 Abstract Molecular methods for microbial community characterization have uncovered environmental and plant-associated factors shaping phyllosphere communities. Variables undetectable using bulk methods can play an …
Microbes in the house Seasonal Dynamics of the Airborne Bacterial Community and Selected Viruses in a Children’s Daycare Center – Aaron J. Prussin II – PLOS ONE (OA) We have investigated the microbial community of the air in a daycare center, including seasonal dynamics in the bacterial community and the presence of specific viral pathogens. …
Another paper on how sample processing (and in this case PCR primer choice) can influence microbiome studies. And another one that is definitely worth looking at: 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice. Microbiome 2015, 3:26 doi:10.1186/s40168-015-0087-4 by Alan W. Walker, Jennifer C. Martin, …
