Tag: V4

If you think your city subway only consists of smelly disgruntled commuters, think again…

By Marcus Leung   Posted in Scholarly Literature (Journals, Books, Reports)
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Note by Jonathan Eisen Last week I saw an interesting new paper in AEM entitled: Indoor-Air Microbiome in an Urban Subway Network: Diversity and Dynamics.  I thought it was of relveance of microBEnet so I wrote to the senior author Dr. Patrick Lee from the School of Energy and Environment and the City University of Hong Kong inviting …

Comparing the new 16S rRNA V4 and ITS primers to the old primers-RESULTS!

By Embriette Hyde   Posted in Bioinformatics Methods and Tools Research from microBEnet
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The Knight lab has been working hard testing new primers for 16S rRNA amplicon production and its time to share our progress. So far, the 16S rRNA V4 region forward primer (designated 515f) has been paired with five different reverse primers (806r, 926r, 967r, 1048r, and 1391r) to amplify ribosomal RNA from bacteria, Archaea, and …