Why genome completeness and contamination estimates are more complicated than you think

Genome-resolved metagenomics is the process of recovering de novo genomes belonging to individual organisms present in a community using DNA extracted from the whole community. These genomes are sometimes referred to as MAGs (metagenome-assembled genomes), and are recovered by associating assembled contigs together in “bins” in a process known as genome binning. As opposed to …

Mars Dome – a cool closed system

According to a recent BBC news article, NASA is doing a Mars simulation project to see if six live human beings can survive being isolated in a dome together. From a MoBE perspective, it would be cool to study this system. It’s completely closed to outside microbes – the people living in this dome have to …

Contaminomics

To all of the microbial researchers out there, be careful about contaminants in your cultures, reagents, and equipment! Yes, we all are careful about good techniques and having proper controls. However, this article suggested that perhaps contaminants are surfacing much more in microbial related literature than we thought. This concept isn’t new, and the aforementioned …

Spinal Tap-The Fungal Meningitis Edition

For anyone looking for another reason to fear hospitals, nosocomial infections (hospital acquired infections), or spinal injections, here is a story for you. Contaminated spinal injections were given to patients in 20 states and led to 751 individuals developing fungal meningitis and 64 deaths. FDA and CDC officials conducted a preliminary investigation and discovered a …

Who are the contaminants in your sequencing project?

Well, been having many discussions recently about PCR amplification happening from “negative” controls where no sample DNA was added. Such amplification is alas pretty common – due to contamination occurring in some other material added to the PCR reaction.  Obviously it would be best to eliminate all DNA contamination of all reagents and all PCRs. …