So I had two thoughts when I read the article title “Indexed PCR Primers Induce Template-Specific Bias in Large-Scale DNA Sequencing Studies“… #1: Ack! Yet another thing to worry about in the insanely complicated craziness that is 16S amplicon sequencing. #2: I probably shouldn’t be the person to blog about this because I’ve honestly never …
When I first started trying to do PCR in Colleen Cavanaugh‘s lab in 1989, I was kind of on my own. Colleen was a newly hired profession at Harvard. She was busy getting things set up. And I was the only person in the lab – and I really knew very little. And basically I …
Another paper on how sample processing (and in this case PCR primer choice) can influence microbiome studies. And another one that is definitely worth looking at: 16S rRNA gene-based profiling of the human infant gut microbiota is strongly influenced by sample processing and PCR primer choice. Microbiome 2015, 3:26 doi:10.1186/s40168-015-0087-4 by Alan W. Walker, Jennifer C. Martin, …
I got this email a few days ago from Jed Fuhrman. He had sent it to a group of people working on mcirobial diversity and he encouraged people to share it. I asked and he approved posting it here and I thought it would be of interest. (The image above is from Lane et al. 1985 …