This is a pre-print that just came out from several UC Davis colleagues. I actually think the title is a bit misleading because these recommendations are a lot broader than just human fecal metagenomes. Definitely worth a read! Abstract below: Background Shotgun metagenomes are often assembled prior to annotation of genes which biases the functional …
(Note: This post was updated on 3/26/18 after re-running some of our analysis based on suggestions in the comments and on Twitter). We were very excited to get our MinION sequencer from Nanopore 1.5 years ago… so many potential applications; sequencing in real time, use in the classroom, use in the field, not having to …
Every year for the last few years I have given a talk on the “Evolution of DNA Sequencing” at the “Workshop in Applied Phylogenetics” at Bodega Bay Marine Lab. I just did the talk and thought I would post the slides here. I note – I also added an evolutionary tree of sequencing methods which …
Microbial ecology has benefited enormously from the development of high throughput sequencing technologies, driving the discovery of massive diversity in environments from the ocean to the human body. Where sequencing of less than one hundred 16S rRNA genes from several samples used to be common place with cloning and Sanger sequencing, we can now generate tens …
I thought this might be of interest to some of the “microbiology of the Built Environment” crowd. I gave a talk at the UC Davis Bodega Bay Marine Lab as part of the Workshop on Applied Phylogenetics on the “Evolution of DNA Sequencing”. I posted the slides to Slideshare and am embedding them here. I …