Metagenomic Binning / Assembly
Posted a request to Twitter about Metagenomic dinners and assemblers and got a lot of useful responses so collected them together into a Wakelet. See below:
Posted a request to Twitter about Metagenomic dinners and assemblers and got a lot of useful responses so collected them together into a Wakelet. See below:
Source: Banfield-lab bioinformatics postdoc announcement – Google Docs Postdoctoral Scholar: Microbial genome bioinformatics – Innovative Genomics Institute, UC Berkeley The Banfield Lab (https://nanogeoscience.berkeley.edu/) and Innovative Genomics Institute (https://innovativegenomics.org/) at the University of California, Berkeley invite applications for a Postdoctoral Researcher position in the field of genome-resolved metagenomics. Our group specializes in generating novel insights …
A colleague has a question they would love some feedback on regarding PCR primer selection for 16S rRNA gene amplicon surveys. I am posting it below: I am working on a project aimed to determine the influence of long-term fertilization on soil microbial communities. I am sampling both the rhizosphere and the bulk soil and …
One of the hurdles in linking microbial ecology with building science has been incorporating quantitative information about the microorganisms encountered in indoor environments, mainly because the standard high-throughput amplicon approach for community analysis is semi-quantitative, at best. Over the summer, there was a Twitter conversation related to this topic. My take-away from this (what I view …
Of potential interest: Source: FICUS JGI-NERSC-KBase – DOE Joint Genome Institute I am copying some of the announcement below: The Biological Data Science call will enable users to perform state-of-the-art computational research to explore the wealth of genomic and metagenomic data generated worldwide and translate sequence information into biological discovery. Users will have access to …
This preprint seems like it may be of interest to folks: Precise prediction of antibiotic resistance in Escherichia coli from full genome sequences | bioRxiv Basically, the authors showed that, using a machine learning approach, they can quite accurately predict antibiotic resistance in E. coli from whole genome data. The emergence of microbial antibiotic resistance …
Twitter Logo: Wikimedia Commons Public Domain Thought this might be of interest to various folks. I made a Twitter moment regarding a discussion about microbial genome annotation methods. Microbial Genome Annotation Methods https://platform.twitter.com/widgets.js
So – just a quick post for now. More details to come. But I wanted to get a little bit about this out there. For a few years I have been teaching a mid-level course at UC Davis on “DNA sequencing based studies of microbial diversity’. The course has evolved over the years from a …
(Note: This post was updated on 3/26/18 after re-running some of our analysis based on suggestions in the comments and on Twitter). We were very excited to get our MinION sequencer from Nanopore 1.5 years ago… so many potential applications; sequencing in real time, use in the classroom, use in the field, not having to …
I entered the world of 16S rRNA gene metabarcoding as a naïve PhD student around five years ago. Like many people, I had hoped that there would have been others before me that had beaten the path and could guide me through the many pitfalls of this experimental approach. How wrong I was! I became …